pmedpharmФармация и фармакологияPharmacy & Pharmacology2307-92662413-2241Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical Univer10.19163/2307-9266-2024-12-1-4-14pmedpharm-1445Research ArticleОРИГИНАЛЬНАЯ СТАТЬЯRESEARCH ARTICLEОценка эффективности серосодержащих аминокислот у крыс с экспериментальным «алкогольным похмельем» с помощью методики зоосоциального взаимодействияEffectiveness assessment of sulfur-containing amino acids in rats with experimental “alcohol withdrawal syndrome” with modified zoosocial interaction methodshttps://orcid.org/0000-0002-0258-4092ПетровВ. И.PetrovV. I.

доктор медицинских наук, профессор, академик РАН, заведующий кафедрой клинической фармакологии и интенсивной терапии, ФГБОУ ВО ВолгГМУ Минздрава России; главный внештатный специалист – клинический фармаколог Министерства здравоохранения РФ; заслуженный деятель науки РФ; заслуженный врач РФ. 

400131, Россия, г. Волгоград, пл. Павших Борцов, д. 1

Doctor of Sciences (Medicine), Professor, Academician of the Russian Academy of Sciences, Head of the Department of Clinical Pharmacology and Intensive Care, Volgograd State Medical University of the Ministry of Health of Russia; chief freelance specialist – clinical pharmacologist of the Ministry of Health of the Russian Federation; Honored Scientist of the Russian Federation; Honored Doctor of the Russian Federation.

1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131

brain@sprintnet.ruhttps://orcid.org/0000-0002-7398-2186ОсадченкоН. А.OsadchenkoN. A.

очный аспирант кафедры клинической фармакологии и интенсивной терапии ФГБОУ ВО ВолгГМУ Минздрава России. 

400131, Россия, г. Волгоград, пл. Павших Борцов, д. 1

full-time postgraduate student of the Department of Clinical Pharmacology and Intensive Care at Volgograd State Medical University.

1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131

n.a.osadchenko@gmail.comhttps://orcid.org/0000-0002-8581-1595ТарасовА. С.TarasovA. S.

ассистент кафедры фундаментальной медицины и биологии ФГБОУ ВО ВолгГМУ Минздрава России; младший научный сотрудник лаборатории нейропсихотропных средств НЦИЛС ФГБОУ ВО ВолгГМУ Минздрава России.

400131, Россия, г. Волгоград, пл. Павших Борцов, д. 1.

400087, Россия, г. Волгоград, ул. Новороссийская, д. 39

Junior Researcher at the Laboratory of Neuropsychotropic Drugs of the Scientific Center for Innovative Medicines of Volgograd State Medical University.

1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131.

39, Novorossiyskaya Str., Volgograd, Russia, 400087.

tarasov.pharm@ya.ruhttps://orcid.org/0000-0003-3324-3351ДоценкоА. М.DotsenkoA. M.

ассистент кафедры фундаментальной медицины и биологии, заочный аспирант кафедры клинической фармакологии и интенсивной терапии ФГБОУ ВО ВолгГМУ Минздрава России; младший научный сотрудник лаборатории фармакокинетики НЦИЛС ФГБОУ ВО ВолгГМУ Минздрава России.

400131, Россия, г. Волгоград, пл. Павших Борцов, д. 1.

400087, Россия, г. Волгоград, ул. Новороссийская, д. 39

Assistant at the Department of Fundamental Medicine and Biology, part-time postgraduate student of the Department of Clinical Pharmacology and Intensive Care at Volgograd State Medical University, Junior Researcher at the Laboratory of Genomic and Proteomic Research of the Scientific Center for Innovative Medicines of Volgograd State Medical University

1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131.

ev8278@mail.ruhttps://orcid.org/0000-0002-7119-3546МорковинЕ. И.MorkovinE. I.

кандидат медицинских наук, доцент, заведующий лабораторией нейропсихофармакологии НЦИЛС ФГБОУ ВО ВолгГМУ Минздрава России.

400087, Россия, г. Волгоград, ул. Новороссийская, д. 39

Candidate of Sciences (Medicine), Associate Professor, Head of the Laboratory of Neuropsychotropic Drugs at the Scientific Center for Innovative Medicines of Volgograd State Medical University.

39, Novorossiyskaya Str., Volgograd, Russia, 400087

e.i.morkovin@gmail.comФедеральное государственное бюджетное образовательное учреждение высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияVolgograd State Medical UniversityRussian Federation1. Федеральное государственное бюджетное образовательное учреждение высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации. 2. Научный центр инновационных лекарственных средств с опытно-промышленным производством федерального государственного бюджетного образовательного учреждения высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации.Россия1. Volgograd State Medical University. 2. Scientific Center of Innovative Medicines.Russian Federation1. Федеральное государственное бюджетное образовательное учреждение высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации. 2. Научный центр инновационных лекарственных средств с опытно-промышленным производством федерального государственного бюджетного образовательного учреждения высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации.РоссияVolgograd State Medical UniversityRussian FederationНаучный центр инновационных лекарственных средств с опытно-промышленным производством федерального государственного бюджетного образовательного учреждения высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияScientific Center of Innovative MedicinesRussian Federation202407052024121414Copyright © Петров В.И., Осадченко Н.А., Тарасов А.С., Доценко А.М., Морковин Е.И., 20242024Петров В.И., Осадченко Н.А., Тарасов А.С., Доценко А.М., Морковин Е.И.Petrov V.I., Osadchenko N.A., Tarasov A.S., Dotsenko A.M., Morkovin E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmpharm.ru/jour/article/view/1445Цель

Цель. Сравнить влияние адеметионина, ацетилцистеина и таурина на зоосоциальное поведение крыс, находящихся в постинтоксикационном состоянии после острого отравления этанолом.

Материалы и методы

Материалы и методы. Исследование проведено на крысах-самцах линии Wistar. Постинтоксикационное состояние моделировали однократным введением этанола (3 г/кг, внутрибрюшинно). Через 30 мин после пробуждения животным вводили: физиологический раствор, ацетилцистеин (1 г/кг), адеметионин (100 мг/кг) или таурин (40 мг/кг). Через 30 мин после введения проводили тест зоосоциального взаимодействия.

Результаты

Результаты. Среди исследуемых показателей поведения были приняты во внимание: количество актов замирания, длительность следования, количество актов обнюхивания спереди, количество актов избегания и количество вертикальных стоек без опоры (p <0,05 между показателями групп отрицательного и положительного контроля во всех случаях). Введение ацетилцистеина, адеметионина и таурина снижало количество актов замирания на 53,64, 7,27 и 24,51% соответственно (p <0,05 при сравнении с показателем у животных из группы положительного контроля во всех случаях). Введение ацетилцистеина и таурина снижало количество актов избегания на 50 и 10% соответственно (p <0,05 при сравнении с показателем у животных из группы положительного контроля в обоих случаях). Все аминокислоты нормализовали показатели коммуникативности, несмотря на то, что они не отличались от показателей у животных из группы положительного контроля (p >0,05). Алкоголизация снижала количество вертикальных стоек на 65% (p <0,001 при сравнении с показателем у животных из группы отрицательного контроля), а при последующем введении адеметионина и таурина снижение составило 38 и 36% соответственно (p <0,05 при сравнении с показателем у животных из группы отрицательного контроля).

Заключение

Заключение. Согласно полученным данным, серосодержащие аминокислоты, в первую очередь те, что обладали центральными эффектами, нормализовали функции нейронов, положительно влияя на сложное поведение крыс. Учитывая результаты предыдущих исследований, удалось заключить, что терапевтическое действие адеметионина и таурина в контексте постинтоксикационного состояния опосредовано их центральными эффектами, не столь выраженными в сравнении с ацетилцистеином.

The aim of the work was to compare the effects of ademethionine, acetylcysteine and taurine on the zoosocial behavior of rats in a post-intoxicated state after an acute ethanol poisoning.

Materials and methods

Materials and methods. The study was conducted on male rats of the Wistar line. The post-intoxication state was modeled by a single injection of ethanol (3 g/kg, intraperitoneally). 30 min after awakening, the animals were injected with a physiological solution, acetylcysteine (1 g/kg), ademetionine (100 mg/kg) or taurine (40 mg/kg). A zoosocial interaction test was performed 30 min after the administration.

Results

Results. Among the behavioral indicators investigated, the following were validated: the number of acts of freezing, their duration, the number of acts of sniffing in front, the number of acts of avoidance and the number of vertical stances without support (p <0.05 between the values of the negative and positive control groups in all cases). The administration of acetylcysteine, ademetionine and taurine reduced the number of freezing acts by 53.64, 7.27 and 24.51%, respectively (p <0.05 when compared with the indicator index in the animals from the positive control group in all cases). The administration of acetylcysteine and taurine reduced the number of avoidance acts by 50 and 10%, respectively (p <0.05 when compared to that of the animals from the positive control group in both cases). All amino acids normalized the communicative performance, although it did not differ from that of the animals from the positive control group (p >0.05). Alcoholization reduced the number of vertical stances by 65% (p <0.001 when compared with that in the animals from the negative control group), and when followed by the administration of ademetionine and taurine, the reduction was 38 and 36%, respectively (p <0.05 when compared to that in the animals from the negative control group).

Conclusion

Conclusion. According to the data obtained, sulfur-containing amino acids, primarily those that had central effects, normalized neuronal functions, positively influencing a complex behavior of rats. Taking into account the results of the previous studies, it was possible to conclude that the therapeutic effect of ademetionine and taurine in the context of a post-intoxication state is mediated by their central effects, which are not so pronounced in comparison with acetylcysteine.

этанолацетилцистеинтауринадеметиониндоклинические исследованияethanolacetylcysteinetaurineademetioninepreclinical studiesДанная работа была выполнена при грантовой поддержке Президента Российской Федерации по Соглашению о предоставлении из федерального бюджета грантов в форме субсидий в соответствии с п. 4 ст. 78.1 Бюджетного кодекса Российской Федерации (внутренний номер МК-3454.2019.7) № 075-15-2019-176 от 23.05.2019 г.This work was performed with the grant support of the President of the Russian Federation under the Agreement on granting grants from the federal budget in the form of subsidies in accordance with Paragraph 4 of Article 78.1 of the Budget Code of the Russian Federation (the internal number MK-3454.2019.7) No. 075-15-2019-176 dated 23.05.2019.Referencesvan Schrojenstein Lantman M., van de Loo A.J., Mackus M., Verster J.C. 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The authors declare that there are no conflicts of interest present.