pmedpharmФармация и фармакологияPharmacy & Pharmacology2307-92662413-2241Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical Univer10.19163/2307-9266-2017-5-5-422-441pmedpharm-268Research ArticleОБЗОРЫ, ЛЕКЦИИREVIEWS, LECTURESОСОБЕННОСТИ КУЛЬТИВИРОВАНИЯ ШТАММОВ HAEMOPHILUS INFLUENZAE ТИП B - ПРОДУЦЕНТОВ ПОЛИРИБОЗИЛРИБИТОЛФОСФАТА - ОСНОВНОГО КОМПОНЕНТА ПОЛИСАХАРИДНЫХ ВАКЦИНPECULIARITIES OF CULTIVATION OF HAEMOPHILUS INFLUENZAE TYPE B STRAINS - PRODUCERS OF POLYRIBOSYLRIBITOL PHOSPHATE - THE MAIN COMPONENT OF POLYSACCHARIDE VACCINESСалимоваЕ. Л.SalimovaE. L.

Салимова Елена Леонидовна – начальник цеха «Комбинированные вакцины».

198320, Санкт-Петербург, Красное Село, ул. Свободы, д. 52

Salimova Elena Leonidovna – head of the department “Combined Vaccines”.

52, Svoboda Str, Krasnoe selo, St.-Peterburg, 198320

e.l.salimova@spbniivs.ruКононА. Д.KononA. D.

Конон Анастасия Дмитриевна – кандидат технических наук, ведущий инженер-технолог цеха «Комбинированные вакцины».

198320, Санкт-Петербург, Красное Село, ул. Свободы, д. 52

Konon Anastasiia Dmitrievna – Candidate of Sciences (Engineering), leading process engineer of the department “Combined Vaccines”.

52, Svoboda Str, Krasnoe selo, St.-Peterburg, 198320

a.d.konon@spbniivs.ruПетровскийС. В.PetrovskiiS. V.

Петровский Станислав Викторович – первый заместитель директора.

198320, Санкт-Петербург, Красное Село, ул. Свободы, д. 52

Petrovskii Stanislav Viktorovich – first deputy director.

52, Svoboda Str, Krasnoe selo, St.-Peterburg, 198320

ТрухинВ. П.TruhinV. P.

Трухин  Виктор  Павлович  –  директор.

198320, Санкт-Петербург, Красное Село, ул. Свободы, д. 52

Truhin Viktor Pavlovich – director.

52, Svoboda Str, Krasnoe selo, St.-Peterburg, 198320

КрасильниковИ. В.KrasilnikovI. V.

Красильников Игорь Викторович – доктор биологических наук, профессор, заместитель директора по международным отношениям.

198320, Санкт-Петербург, Красное Село, ул. Свободы, д. 52

Krasilnikov Igor Viktorovich – Doctor of Sciences (Biology), professor deputy director for international relations.

52, Svoboda Str, Krasnoe selo, St.-Peterburg, 198320

i.v.krasilnikov@spbniivs.ruФедеральное государственное унитарное предприятие «Санкт-Петербургский научно-исследовательский институт вакцин и сывороток и предприятие по производству бактерийных препаратов» Федерального медико-биологического агентстваРоссияThe federal state unitary enterprise “Saint-Petersburg scientific research institute of vaccines and serums and the enterprise for the production of bacterial preparations” of Federal medical and biologic agencyRussian Federation20173010201755422441Copyright © Салимова Е.Л., Конон А.Д., Петровский С.В., Трухин В.П., Красильников И.В., 20172017Салимова Е.Л., Конон А.Д., Петровский С.В., Трухин В.П., Красильников И.В.Salimova E.L., Konon A.D., Petrovskii S.V., Truhin V.P., Krasilnikov I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmpharm.ru/jour/article/view/268

Одной из актуальных задач современной иммунобиотехнологии является разработка и внедрение эффективной вакцины против гемофильной инфекций, возбудителем которой является бактерия Haemophilus influenzae тип b (Hib). Основным действующим веществом вакцины против Hib-инфекции является капсульный полисахарид полирибозилрибитолфосфат (ПРФ), который выделяют из культуральной жидкости H. influenzae тип b. Важным технологическим этапом получения ПРФ является культивирование штамма-продуцента в условиях, позволяющих получить максимальное количество целевого продукта. На данный момент планируется подбор оптимальных условий культивирования штамма H. influenzae тип b В-7884, изолированного и идентифицированного ранее сотрудниками ФГУП «Санкт-Петербургский научно-исследовательский институт вакцин и сывороток и предприятие по производству бактерийных препаратов» ФМБА России.

Цель данной работы – обобщить и проанализировать данные литературы относительно особенностей культивирования штаммов H. influenzae тип b, а также выделения основных факторов, влияющих на биосинтез ПРФ.

Материалы и методы

Материалы и методы. В процессе подбора материала для написания обзорной статьи использовали базы данных Google Patents, Science Research Portal, Google Scholar, ScienceDirect, CiteSeer, Publications, ResearchIndex, Ingenta, PubMed, KEGG и др.

Результаты и обсуждение

Результаты и обсуждение. В результате анализа литературы были выделены основные факторы, влияющие на биосинтез ПРФ: природа и концентрация источников углерода и азота в составе питательной среды, концентрация факторов роста (никотиамидадениндинуклеотид, гемин, витамины), дополнительное внесение подпитки, регуляция рН в процессе культивирования, скорость перемешивания. Обобщены данные по штаммам-продуцентам ПРФ и условиям их культивирования, а также количеству синтезированного ПРФ, которое существенно зависит и от физиологических возможностей биологического агента, и от факторов, влияющих на регуляцию метаболизма.

Заключение

Заключение. Результаты данной работы будут учитываться при проведении исследований по оптимизации условий культивирования штамма H. influenzae тип b В-7884.

One of the up-to-date challenges of modern immunobiotechnology is the development and introduction of an effective vaccine against the infection caused by the bacterium Haemophilus influenza, type b (Hib). The main active substance of the vaccine against Hib infection is the capsular polysaccharide polyribosylribitol phosphate (PRP), which is isolated from the fermentation broth of H. influenzae type b. An important technological step in obtaining PRP is the cultivation of the producer strain under conditions that allow obtaining the maximum amount of the target product. At the moment, it is planned to select the optimal conditions for cultivation of H. influenzae type b B-7884, which had been earlier isolated and identified by the employees of FSUE “Saint-Petersburg scientific research institute of vaccines and serums and the enterprise for the production of bacterial preparations” of FMBA, Russia. The analysis of literature data concerning the cultivation of H. influenzae type b was made in order to identify the main factors influencing the biosynthesis of PRP.

The aim of the investigation is to analyze and summarize the literature data on the cultivation peculiarities of haemophilus influenzae of b-type strains.

Materials and methods

Materials and methods. In the process of selecting the material for writing this review article. The databases of Google Patents, Science Research Portal, Google Scholar, ScienceDirect, CiteSeer Publications ResearchIndex, Ingenta, PubMed, KEGG, etc. were used.

Results and discussion

Results and discussion. As a result of the literature analysis, the main factors influencing the PRP biosynthesis were identified: the nature and concentration of carbon and nitrogen sources in the growth medium, the concentration of growth factors (nicotinamide adenine dinucleotide, hemin, vitamins), additional feed, pH adjustment during cultivation, stirring speed. The data of PRP-producing strains and the conditions of their cultivation have been summarized, as well as the amount of synthesized PRP, which essentially depends on both the physiological capabilities of the biological agent and the factors effecting the regulation of metabolism.

Conclusion

Conclusion. The results of this work will be taken into account in carrying out the researches for optimization of H. influenzae type b B-7884 strain cultivation conditions.

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The authors declare that there are no conflicts of interest present.