pmedpharmФармация и фармакологияPharmacy & Pharmacology2307-92662413-2241Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical Univer10.19163/2307-9266-2019-7-3-129-137pmedpharm-396Research ArticleФАРМАЦЕВТИЧЕСКАЯ ТЕХНОЛОГИЯ И БИОТЕХНОЛОГИЯPHARMACEUTICAL TECHNOLOGY AND BIOTECHNOLOGYИЗУЧЕНИЕ КИНЕТИКИ ГИДРОЛИЗА БАЙКАЛИНА ПРИ ЕГО ЭКСТРАКЦИИ ИЗ КОРНЕЙ ШЛЕМНИКА БАЙКАЛЬСКОГОSTUDY OF BAICALIN HYDROLYSIS KINETICS IN THE PROCESS OF ITS EXTRACTION FROM SCUTELLARIA BAICALENSIS GEORGI ROOTShttps://orcid.org/0000-0001-9222-2935БойкоН. Н.BoykoN. N.

кандидат фармацевтических наук, доцент, младший научный сотрудник лаборатории технологии лекарств, доцент кафедры фармацевтической технологии,

308015, г. Белгород, ул. Победы, 85

PhD (Pharmacy), Junior Researcher of Drug Technology Laboratory, Associate Professor of Pharmaceutical Technology Department,

85, Pobedа St., Belgorod, 308015

boykoniknik@gmail.comhttps://orcid.org/0000-0002-2996-7712ПисаревД. И.PisarevD. I.

доктор фармацевтических наук, доцент, профессор кафедры общей химии,

308015, г. Белгород, ул. Победы, 85

PhD (Pharmacy), Associate Professor, Professor of General Chemistry Department,

85, Pobedа St., Belgorod, 308015

pisarev@bsu.edu.ruЖиляковаЕ. Т.ZhilyakovaE. T.

доктор фармацевтических наук, профессор, заведующая кафедрой фармацевтической технологии,

308015, г. Белгород, ул. Победы, 85

PhD (Pharmacy), Professor, Head of Pharmaceutical Technology Department,

85, Pobedа St., Belgorod, 308015

ezhilyakova@bsu.edu.ruhttps://orcid.org/0000-0001-6170-2151МалютинаА. Ю.MaljutinaA. Yu.

кандидат фармацевтических наук, доцент кафедры общей химии,

308015, г. Белгород, ул. Победы, 85

PhD (Pharmacy), Associate Professor of General Chemistry Department,

85, Pobedа St., Belgorod, 308015

malyutina_a@bsu.edu.ruhttps://orcid.org/0000-0003-3145-6783НовиковО. О.NovikovO. O.

доктор фармацевтических наук, профессор, заведующий Центра коллективного пользования (научно-образовательный центр) «Центр контроля качества лекарств», 

117198, г. Москва, ул. Миклухо-Маклая, 8/2

PhD (Pharmacy), Professor, Head of the Center for Collective Use (Research and Education Center) “Center for Quality Control of Drugs”,

8/2, Miklukho-Maklay St., Moscow, 117198

ole9222@yandex.ruhttps://orcid.org/0000-0003-3524-848XБочарниковаМ. А.BocharnikovaM. A.

аспирант 3 года обучения кафедры общей химии,

308015, г. Белгород, ул. Победы, 85

3-year postgraduate student of General Chemistry Department,

85, Pobedа St., Belgorod, 308015

bocharnikova_m@bsu.edu.ruФедеральное государственное автономное образовательное учреждение высшего образования научно-исследовательский университет «Белгородский государственный университет» Министерства образования и науки РФРоссияBelgorod National Research UniversityRussian FederationФедеральное государственное автономное образовательное учреждение высшего образования «Российский университет дружбы народов»РоссияPeoples’ Friendship University of RussiaRussian Federation20190907201973129137Copyright © Бойко Н.Н., Писарев Д.И., Жилякова Е.Т., Малютина А.Ю., Новиков О.О., Бочарникова М.А., 20192019Бойко Н.Н., Писарев Д.И., Жилякова Е.Т., Малютина А.Ю., Новиков О.О., Бочарникова М.А.Boyko N.N., Pisarev D.I., Zhilyakova E.T., Maljutina A.Y., Novikov O.O., Bocharnikova M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmpharm.ru/jour/article/view/396Цель

Цель. Изучить кинетику гидролиза байкалина при его экстракции из корней шлемника байкальского.

Материалы и методы

Материалы и методы. Корни шлемника байкальского с размером частиц 0,1-0,5 мм. Используемый метод экстракции – простая мацерация в течение заданного промежутка времени, при соотношении сырье: экстрагент 1:10 м/о и температуре 24±1°С. Содержание байкалина и байкалеина анализировали с помощью обратно-фазовой высокоэффективной жидкостной хроматографии (ОФ ВЭЖХ) при длине волны 275 нм. Экстрагент: водные растворы этанола 26, 43, 59, 72, 81, 97±1% об. Время настаивания от 1 до 24 часов.

Результаты

Результаты. Экспериментальные точки зависимости концентрации байкалина в извлечении от времени настаивания для этанола с концентрацией 43 и 72% об., хорошо аппроксимируются линейным уравнением в координатах lnC=f(t). Коэффициент детерминации боле R²˃0,99. Рассчитано время полураспада байкалина в этаноле с концентрацией 43 % об., которое составило 4,6±0,5 часа, в этаноле с концентрацией 72% об., данный показатель равен 42,3±1,8 часа.

Заключение

Заключение. Изучена кинетика гидролиза байкалина при его экстракции из корней шлемника байкальского с помощью этанола с концентрацией 43 и 72% об. Установлено, что процесс гидролиза байкалина хорошо описывается кинетическим уравнением первого порядка. Найдены константы процесса гидролиза байкалина во время его экстракции из корней шлемника байкальского с помощью этанола различной концентрации. Даны рекомендации по оптимизации технологии выделения байкалина или байкалеина из корней шлемника байкальского. 

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots.

Materials and methods

Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1–0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°С. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours.

Results

Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R²˃0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours.

Conclusion

Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given. 

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The authors declare that there are no conflicts of interest present.