pmedpharmФармация и фармакологияPharmacy & Pharmacology2307-92662413-2241Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical Univer10.19163/2307-9266-2019-7-6-279-290pmedpharm-547Research ArticleФАРМАКОГНОЗИЯ, БОТАНИКАPHARMACOGNOSY, BOTANYФитохимическое исследование травы якорцев стелющихсяPhytochemical study of Tribulus Terrestris L.АффуфА.AffafA.

Аффуф Абдулкарим – аспирант кафедры фармакогнозии с курсом ботаники 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Abdulkarim Affuf – post-graduate student of the Department of Pharmacognosy with a botany course 

2, Polevaya Str., Perm, Russia 614990

aboud.bashar89@gmail.comКарпенкоЮ. Н.KarpenkoYu. N.

Карпенко Юлия Николаевна – кандидат фармацевтических наук, доцент кафедры токсикологической химии 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Julia N. Karpenko – Candidate of Sciences (Pharmacy), Associate Professor of the Department of Toxicological Chemistry

2, Polevaya Str., Perm, Russia 614990

Karpenko@pfa.ruhttps://orcid.org/0000-0001-9464-1869ГуляевД. К.GulyaevD. K.

Гуляев Дмитрий Константинович – кандидат фармацевтических наук, старший преподаватель кафедры фармакогнозии с курсом ботаники 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Dmitry K. Gulyaev – Candidate of Sciences (Pharmacy), Senior Lecturer in the Department of Pharmacognosy with a course in Botany

2, Polevaya Str., Perm, Russia 614990

dkg2014@mail.ruБелоноговаВ. Д.BelonogovaV. D.

Белоногова Валентина Дмитриевна – доктор фармацевтических наук, профессор, заведующий кафедрой фармакогнозии с курсом ботаники

614990, Россия, г. Пермь, ул. Полевая, д. 2

Valenti D. Belonogova – Doctor of Sciences (Pharmacy), Professor, Head of the Department of Pharmacognosy with a Botany Course

2, Polevaya Str., Perm, Russia 614990

belonogova@pfa.ruМолоховаЕ. И.MolokhovaE. I.

Молохова Елена Игоревна – доктор фармацевтических наук, профессор кафедры промышленной технологии с курсом биотехнологии 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Elena I. Molokhova – Doctor of Sciences (Pharmacy), Professor of the Department of Industrial Technology with a biotechnology course

2, Polevaya Str., Perm, Russia 614990

molohova@pfa.ruБлиноваО. Л.BlinovaO. L.

Блинова Ольга Леонидовна – кандидат фармацевтических наук, доцент кафедры фармакогнозии с курсом ботаники 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Olga L. Blinova – Candidate of Sciences (Pharmacy), Associate Professor of the Department of Pharmacognosy with a botany course

2, Polevaya Str., Perm, Russia 614990

oblinova@mail.ruГилеваА. А.GilevaA. A.

Гилева Ангелина Александровна – кандидат фармацевтических наук, доцент кафедры фармакогнозии с курсом ботаники 

614990, Россия, г. Пермь, ул. Полевая, д. 2

Angelina A. Gileva – Candidate of Sciences (Pharmacy), Associate Professor of the Department of Pharmacognosy with a botany course

2, Polevaya Str., Perm, Russia 614990

angelinaustinova@mail.ruФГБОУ ВО «Пермская государственная фармацевтическая академия» Министерства здравоохранения Российской ФедерацииРоссияPerm State Pharmaceutical AcademyRussian Federation20191501202076346355Copyright © Аффуф А., Карпенко Ю.Н., Гуляев Д.К., Белоногова В.Д., Молохова Е.И., Блинова О.Л., Гилева А.А., 20202020Аффуф А., Карпенко Ю.Н., Гуляев Д.К., Белоногова В.Д., Молохова Е.И., Блинова О.Л., Гилева А.А.Affaf A., Karpenko Y.N., Gulyaev D.K., Belonogova V.D., Molokhova E.I., Blinova O.L., Gileva A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmpharm.ru/jour/article/view/547

Якорцы стелющиеся, или якорцы наземные – Tríbulus terrestris L., семейство парнолистниковые – Zygophyllaceae, однолетнее травянистое растение, произрастающее в умеренном и тропическом климате, имеет богатый химический состав биологически активных веществ и химических элементов.

Цель работы – фитохимическое исследование якорцев стелющихся, произрастающих в разных географических зонах.

Материалы и методы

Материалы и методы. Объектами исследования являлись образцы травы якорцев стелющихся, собранные в различных условиях обитания. Образцы сырья были высушены воздушно-теневым способом. Определение сапонинов в сырье проводили методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием (ВЭЖХ-МС/МС). Изучение качественного и количественного состава элементов проводили на рентгенофлуоресцентном спектрометре.

Результаты

Результаты. Проведено исследование сапонинов методом ВЭЖХ-МС/МС, по результатам которого, во всех исследуемых образцах обнаружены диосцин и протодиосцин, времена удерживания которых совпадают с временами удерживания стандартов диосцина и протодиосцина. Установлено, что в образцах якорцев стелющихся травы среди макроэлементов в наибольшем количестве накапливаются калий и кальций, на их долю приходится около 90% от общего содержания элементов в растении. Выявлено, что распределение макро и микроэлементов в растении существенно различается в зависимости от места и условий произрастания.

Заключение

Заключение. Максимальное содержание диосцина наблюдали в образцах, заготовленных в Молдове, а минимальное – в образцах из питомника ФГБНУ ВИЛАР. Наибольшее количество протодиосцина обнаружено в образцах из Крыма, а минимальное – в образцах из Молдовы. Проведенное исследование элементного состава якорцев стелющихся травы показало, что места обитания (географические зоны), в которых осуществлялся сбор исследованных образцов сырья, влияют на накопление элементов растением. С учетом полученных данных составлены ряды биологического поглощения для образцов из каждого места произрастания.

Tríbulus terrestris L

Tríbulus terrestris L., an annual herb belonging to the Zygophyllaceae family and growing in moderate and tropical climates, has a rich chemical composition of biologically active substances and chemical elements.

The aim of the work is a phytochemical study of Tribulus terrestris L. growing in different geographical zones.

Materials and methods

Materials and methods. The objects of study were herb specimens of Tribulus terrestris L. collected in different habitats. The samples of the raw materials were shade-dried. The determination of saponins in the raw materials, was carried out by high performance liquid chromatography with a mass spectrometric detection (HPLC-MS / MS). The study of the qualitative and quantitative composition of the elements was carried out on an X-ray fluorescence spectrometer.

Results

Results. The saponins had been studied by HPLC-MS/MS, according to which in all the studied samples, dioscin and protodioscin were found. Their retention times coincided with the retention times of dioscin and protodioscin standards. It has been established that among the macroelements of Tríbulus terrestris L., potassium and calcium are mostly accumulated. They account for about 90% of the total content of the elements in the plant. It has been revealed that the distribution of macroand microelements in the plant, varies significantly depending on their place and growing conditions.

Conclusion

Conclusion. The maximum dioscin content was observed in the samples harvested in Moldova, and the minimum – in the samples from the nursery garden of the All-Russian Scientific Research Institute of medicinal and aromatic plants. The largest amount of protodioscin was found out in the samples from the Crimea, and the minimum – in the samples from Moldova. The carried out study of the elements content of Tríbulus terrestris L. showed that the habitats (geographical zones) in which the studied samples of raw materials had been were collected, affect the accumulation of the elements by the plant. Based on the data obtained, biological absorption series have been compiled for the samples from each habitat.

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The authors declare that there are no conflicts of interest present.