pmedpharmФармация и фармакологияPharmacy & Pharmacology2307-92662413-2241Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical Univer10.19163/2307-9266-2021-9-6-506-518pmedpharm-982Research ArticleФАРМАКОГНОЗИЯ, БОТАНИКАPHARMACOGNOSY, BOTANYИСПОЛЬЗОВАНИЕ МЕТОДОВ СЕКВЕНИРОВАНИЯ ДЛЯ ИДЕНТИФИКАЦИИ ВИДОВ НА ПРИМЕРЕ ФИЛОГЕНЕТИЧЕСКИХ СВЯЗЕЙ В ПРЕДЕЛАХ РОДА HEDYSARUM L.USE OF SEQUENCING METHODS FOR SPECIES IDENTIFICATION EXEMPLIFIED BY PHYLOGENETIC RELATIONSHIPS WITHIN GENUS HEDYSARUM L.https://orcid.org/0000-0002-8953-3158ИмачуеваД. Р.ImachuevaD. R.

кандидат фармацевтических наук, ассистент кафедры фармации

Candidate of Sciences (Pharmacy), Assistant of the Department of Pharmacy

djakag01@gmail.comhttps://orcid.org/0000-0001-9409-9344СеребрянаяФ. К.SerebryanayaF. K.

кандидат фармацевтических наук, доцент кафедры фармакогнозии, ботаники и технологии фитопрепаратов 

Candidate of Sciences (Pharmacy), Associate Professor of the Department of Pharmacognosy, Botany and Technology of Phytopreparations

f.k.serebryanaya@pmedpharm.ruhttps://orcid.org/0000-0001-9347-5379МачсЭ. М.MachsE. M.

кандидат биологических наук, старший научный сотрудник лаборатории биосистематики и цитологии

Candidate of Sciences (Biology), Senior Researcher, Laboratory of Biosystematics and Cytology

edw.mach@gmail.comhttps://orcid.org/0000-0003-1872-2223КоцерубаВ. В.KotserubaV. V.

кандидат биологических наук, старший научный сотрудник лаборатории биосистематики и цитологии

Candidate of Sciences (Biology), Senior Researcher, Laboratory of Biosystematics and Cytology

VKotseruba@binran.ruФедеральное государственное бюджетное образовательное учреждение высшего образования «Дагестанский государственный медицинский университет» Министерства здравоохранения Российской Федерации 367000, Россия, Республика Дагестан, г. Махачкала, пл. Ленина 1РоссияDagestanian State Medical University 1, Lenin Sq., Makhachkala, Republic of Dagestan, Russia, 367000Russian FederationПятигорский медико-фармацевтический институт – филиал федерального государственного бюджетного образовательного учреждения высшего образования «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации 357532, Россия, Ставропольский край, г. Пятигорск, пр-кт Калинина, 11РоссияPyatigorsk Medical and Pharmaceutical Institute – a branch of Volgograd State Medical University 11, Kalinin Avе., Pyatigorsk, Russia, 357532Russian FederationФедеральное государственное бюджетное учреждение науки Ботанический институт им. В.Л. Комарова Российской академии наук 197376, Россия, г. Санкт-Петербург, ул. Проф. Попова, 2РоссияKomarov Botanical Institute of the Russian Academy of Sciences 2, Prof. Popov Str., St. Petersburg, Russia, 197376Russian Federation20211012202196506518Copyright © Имачуева Д.Р., Серебряная Ф.К., Мачс Э.М., Коцеруба В.В., 20212021Имачуева Д.Р., Серебряная Ф.К., Мачс Э.М., Коцеруба В.В.Imachueva D.R., Serebryanaya F.K., Machs E.M., Kotseruba V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmpharm.ru/jour/article/view/982

Актуальной задачей в фармакогнозии на данный момент является применение всевозможных методов ДНК-анализа для идентификации растительного сырья, выявления фальсификатов, генетически модифицированных сельскохозяйственных культур и продуктов.

Цель

Цель. Изучить возможность применения молекулярно-генетических методов исследований при анализе рода Hedysarum L. для идентификации лекарственного растительного сырья. В данной статье представлены результаты применения молекулярно-генетических методов исследования при анализе рода Hedysarum L. флоры Северного Кавказа.

Материалы и методы

Материалы и методы. Материалом для исследования послужили образцы видов рода Hedysarum L., собранные на территории Северного Кавказа: Hedysarum caucasicum M.Bieb. (фаза плодоношения на территории Карачаево-Черкесской Республики); Hedysarum grandiflorum Pall. (фаза плодоношения в Волгоградской области); Hedysarum daghestanicum Rupr. ex Boiss. (фаза цветения в Республике Дагестан). Секвенирование маркерного участка ITS1-5.8S-ITS2 гена 5.8S рибосомой РНК проводили по методу Сэнгера на генетическом анализаторе AbiPrism 3130 на базе лаборатории биосистематики и цитологии Ботанического института имени В.Л. Комарова РАН.

Результаты

Результаты. На основе сравнительного изучения маркерного участка ядерного рибосомного гена 5.8S рРНК были идентифицированы маркерные нуклеотидные замены Hedysarum caucasicum M. Bieb., Hedysarum daghestanicum Rupr. ex Boiss., Hedysarum grandiflorum Pall. Была построена наиболее вероятная вторичная структура 5.8S рРНК.

Показано, что на основании проведенного анализа можно составить прогноз дополнительных сырьевых источников мангиферина и других групп ксантонов при использовании молекулярных данных, на примере секции Obscura.

Заключение

Заключение. На основании полученных данных можно сделать заключение о том, что в пределах секции Obscura можно подтвердить морфологическую классификацию рода Hedysarum L.

At the moment, a relevant objective in pharmacognosy, is the use of all kinds of the DNA analysis methods for identifying plant materials, detecting counterfeits, genetically modified crops and products.

The aim of the research is to study the possibility of using molecular genetic research methods in the analysis of the genus Hedysarum L., for the identification of medicinal plant materials. This article presents the results of the application of molecular genetic research methods in the analysis of the genus Hedysarum L. in the flora of the North Caucasus.

Materials and methods

Materials and methods. The study material was the samples of the genus Hedysarum L. species collected in the North Caucasus: Hedysarum caucasicum M. Bieb. (in the fruiting phase in the territory of the Karachay-Cherkess Republic); Hedysarum grandiflorum Pall. (in the fruiting phase in the Volgograd region); Hedysarum daghestanicum Rupr. ex Boiss. (in the flowering phase in the Republic of Dagestan).  Sequencing of the ITS1-5.8S-ITS2 marker region of gene 5.8S by the RNA ribosome was carried out according to the Sanger method on the AbiPrism 3130 genetic analyzer at the laboratory of biosystematics and cytology of Komarov Botanical Institute of the Russian Academy of Sciences.

Results

Results. Based on a comparative study of the marker region of the nuclear ribosomal gene 5.8S rRNA, marker nucleotide substitutions of Hedysarum caucasicum M. Bieb., Hedysarum daghestanicum Rupr. ex Boiss., Hedysarum grandiflorum Pall, have been identified. The most probable secondary structure of 5.8S rRNA has been constructed. It has been shown that based on the analysis performed, it is possible to predict additional raw material sources of mangiferin and other groups of xanthones using the molecular data exemplified by the Obscura section.

Conclusion

Conclusion. Based on the data obtained, it can be concluded that the morphological classification of the genus Hedysarum L. can be confirmed within the Obscura section.

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The authors declare that there are no conflicts of interest present.