THE INFLUENCE OF HELICHRYSUM ARENARIUM FLOWERS INFUSION ON MAST CELLS DEGRANULATION IN VITRO

Helichrysum arenarium flowers are medical plant raw materials with a high concentration of flavonoids (2.5– 5.0%). As compounds of this group of biologically active substances often possess antiallergic activity, Helichrysum arenarium flowers are a potential antiallergic medicine. The aim is to study an antiallergic activity of Helichrysum arenarium flowers infusion on the model of mast cells degranulation in vitro. Materials and methods. Helichrysum arenarium flowers infusion was made in accordance with the requirements of the State Pharmacopoeia of the Republic of Belarus. Mast cells were obtained from the abdominal cavity of inbred mice weighing 20–25 g. Experimental animals were divided into three groups of 5 individuals in each. The intact group was not subjected to any manipulation for 14 days. The test group was sensitized with the epidermal allergen from the cat’s fur. The placebo group was subjected to the same manipulations as the test group, by phosphate buffered saline pH 7.4 for sensitization instead of the allergen. Results and discussion. The addition of Helichrysum arenarium flowers infusion to the mast cells of the intact group and the placebo group did not affect the percentage of the mast cells degranulation (p>0.05), indicating that the animals of the intact group and the placebo group did not have a hypersensitivity to the components of the medicine under investigation. In the presence of Helichrysum arenarium flowers the percentage of the mast cells degranulation of the test group decreased in comparison with the percentage of the mast cells degranulation in the presence of the allergen from 23.6±2.1% to 8.1–16.2% (p<0.05). Semi-effective concentration of Helichrysum arenarium flowers infusion was 1.33±0.04 mg / ml. The stabilizing effect on the mast cells degranulation of the Helichrysum arenarium flowers infusion was statistically significantly lower than the stabilizing effect of the Bidens tripartita herb infusion. Conclusion. Helichrysum arenarium flowers infusion has a pronounced dose-dependent stabilizing effect on the mast cells of male mice in vitro. Nevertheless, the stabilizing effect of Helichrysum arenarium flowers infusion on the mast cells was less pronounced than the stabilizing effect of Bidens tripartita herb infusion.

1. Czinner E, Hagymási K, Blázovics A, Kéry A, Szoke E, Lemberkovics E. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation. J. Ethnopharmacol. 2001;1(77):31–5. DOI: 10.1016/S0378-8741(01)00258-6

2. Czinner Е, Hagymási К, Blázovics А, Kéry А, Szőke Е, Lemberkovics Е. In vitro antioxidant properties of Helichrysum arenarium (L.) Moench. J. Ethnopharmacology. 2000;3(73):437–43. DOI: 10.1016/S0378-8741(00)00304-4

3. Tepe В, Sokmen М, Akpulat НА, Sokmen А. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chemistry. 2005;90(4):685–9. DOI: 10.1016/j.foodchem.2004.04.030

4. Kurchatova MN, Larina AS, Andreeva NV. Vliyanie ekstrakta bessmertnika peschanogo na autointoksikaciyu v plazme krovi krys [Influence of the extract of Helichrysum arenarium extract on autointoxication in the rats blood plasma]. Bulletin of Medical Internet Conferences. 2015;5(5):819. Russian.

5. Ivlichev AV, Mudrak DA, Navolokin NA, Afanasyeva GA, Tychina SA, Korchakov MO, Polukonova NV, Bu-charskaya AB, Maslyakova GN. Aktivnost’ perekisnogo okisleniya lipidov pri peroral’nom vvedenii flavonoidsoderzhashchego ekstrakta bessmertnika peschanogo na fone perevivaemogo raka pecheni RS-1 [Activity of lip-ides peroxidation at oral usage of flavonoid-containing Helichrysum arenarium extract on the background of the liver-carcinated cancer RS-1]. Rossiysky Bioterapevtichesky Zhurnal. 2016;1(15):42–3. Russian.

6. Moricawa T, Ninomiya K, Akaki J, Kakihara N, Kuramoto H, Matsumoto Y, Hayakawa T, Muraoka O, Wang L-B, Wu L-J, Nakamura S, Yoshikawa M, Matsuda H. Dipeptidyl Peptidase-IV inhibitory activity of dimeric dihydrochalcone glycosides from flowers of Helichrysum arenarium. J. Natural Medicines. 2015;69(4):494–506. DOI: 10.1007/s11418-015-0914-8

7. Skvortsova VV, Navolokin NA, Polukonova NV, Manaenkova EV, Pankratova LÉ, Kurchatova MA, Masliakova GN, Durnova NA. Antituberculous in vitro activity of Helichrysum arenarium extract. Eksp Klin Farmakol. 2015;78(2):30–3.

8. Gradinaru AC, Silion M, Trifan A, Miron A, Aprotosoaie AC. Helichrysum arenarium subsp. arenarium: phenolic composition and antibacterial activity against lower respiratory tract pathogens. Nat Prod Res. 2014;28(22):2076–80. DOI: 10.1080/14786419.2014.924931

9. Skvortsova VV, Navolokin NA, Polukonova NV, Manaenkova EV, Pankratova LE, Kurchatova MN, Maslyakova GN, Durnova NA. Protivotuberkuleznaya aktivnost’ ekstrakta bessmertnika peschanogo (Helichrysum arenarium) in vitro [Antituberculous in vitro activity of Helichrysum arenarium Extract]. Experimental and Clinical Pharmacology. 2015;2(78):30–3. Russian.

10. Navolokin NA, .Mudrak DA, Polukonova NV, Tychina SA, Kanayeva TV, Bucharskaya AB, Maslyakova GN. Antikaheksicheskaya i protivoopuholevaya aktivnost’ flavonoidsoderzhashchego ekstrakta bessmertnika peschanogo (Helichrusum arenarium) pri peroral’nom vvedenii krysam s perevitoj sarkomoj-45 [Anticaxoxic and antitumoric activity of flavonide-containing Helichrysum arenarium extract in parametric introduction to rats with sarcom-45 insertion] // Malignant tumours. 2016;1(21):329–30. Russian.

11. Bryksa-Godzisz M, Węglarz Z, Przybył J. Phenolic compounds in yellow everlasting (Helichrysum arenari-um (L.) Moench) growing wild in the middle part of the bug river valley. Herba Polonica. 2006;4(52):26–31.

12. Litvinenko VI, Popova TP, Popova NV, Georgievsky VP. K voprosu ob izosalipurpozide-standarte v kontrole syr’ya i fitopreparatov iz bessmertnika peschanogo [To the question of the isosalipurposid-standard in the control of raw material and phytopreparates from the Helichrysum arenarium]. Journal ’Pharmacom’. 2016;3:23–7. Russian.

13. Kurkina AV. Issledovanie komponentnogo sostava cvetkov Helichrysum arenarium (L.) Moench. [Investigation of component composition of Helichrysum arenarium (L.) Moench flowers]. Chemistry of plant raw materials. 2011;2:113–6. Russian.

14. Cubukcu B. Helichrysum species as choleretic and chologogue crude extract. Acta Pharmaceutica Turcica. 2002;44:145–50.

15. Tarasenko YaV, Karazhan NV. Soderzhanie flavonoidov v cvetkah bessmertnika peschanogo [Contents of flavonides in Helichrysum arenarium flowers]. Aktual’nye voprosy sovremennoj mediciny i farmacii»: materialy 69-oj itogovoj nauchno-prakticheskoj konferencii studentov i molodyh uchenyh / VSMU. Vitebsk, 2017:680–1. Russian.

16. Park HH, Lee S, Son HY, Park SB, Kim MS, Choi EJ, Singh TS, Ha JH, Lee MG, Kim JE, Hyun MC, Kwon TK, Kim YH, Kim SH. Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Archives of Pharmacal Research. 2008;31(10):1303–11. DOI: 10.1007/s12272-001-2110-5

17. Mastuda Н, Morikawa Т, Ueda К, Managi Н, Yoshikawa М. Structural requirements of flavonoids for inhibition of antigen-induced degranulation, TNF-Α and IL-4 production from RBL-2H3 cells. Bioorganic & Medicinal Chemistry. 01 Oct 2002;10(10):3123–8. DOI: 10.1016/S0968-0896(02)00227-4

18. Tanaka T, Higa S, Hirano T, Kotani M, Matsumoto M, Fujita A, Kawase I. Flavonoids as potential anti-allergic substances. Current Medicinal Chemistry – Anti-Inflammatory & Anti-Allergy Agents. 2003;2(1):57–65. DOI: 10.2174/1568014033355790

19. Kawai M, Hirano T, Higa S, Arimitsu J, Maruta M, Kuwahara Y, Ohkawara T, Hagihara K, Yamadori T, Shima J, Ogata A, Kawase I, Tanaka T. Flavonoids and related compounds as anti-allergic substances. Allergology International. 2007;56(2):113–23. DOI: 10.2332/allergolint.R-06-135

20. Kimata M, Inagaki N, Nagai H. Effects of luteolin and other flavonoids on IgE-mediated allergic reactions. Planta Med. 2000;66(1):25–9. DOI: 10.1055/s-2000-11107

21. Dai Y, Hou LF, Chan YP, Cheng L, But PPH. Inhibition of immediate allergic reactions by ethanol extract from Plumbago zeylanica stems. Biological and Pharmaceutical Bulletin. 2004;27(3):429–32. DOI: 10.1248/ bpb.27.429

22. Vykhrystsenka LR, Novikov DK, Yanchanka UV. Issledovanie bezopasnosti i effektivnosti peroral’nyh nizkodozovyh allergovakcin dlya lecheniya bronhial’noj astmy [Study the safety and efficacy of oral low dose allergovaccines for the treatment of bronchial asthma]. International Journal of Immunopathology, Allergology, Infectology. 2011;2:70–80. Russian.

23. Suyarov AA, Japorov OK, Hatamov HM. Ocenka protivoallergicheskoj effektivnosti summy flavonoidov, vydelennoj iz cheredy trekhrazdel’noj v raznyh dozah pri eksperimental’noj kozhnoj allergii [The estimation anti-allergic efficiency of the amount flavonoidov, chosen from Bidens tripartitae in different dose under experimental skin allergy]. Fiziologiya i patologiya immunnoj sistemy. Immunofarmakogenomika. 2014;1:48–52. Russian.

24. Karazhan NV, Buzuk GN. Stabiliziruyushchee dejstvie na membrany tuchnyh kletok travy cheredy trekhrazdel’noj i travy cheredy olistvennoj [Stabilizing action on the membranes of mast cells of Bidens tripartita herb and Bidens frondosa herb]. Vestnik farmacii. 2015;1:136–43. Russian.

25. Karazhan NV, Buzuk GN. Sravnitel’nyj analiz komponentnogo sostava spirtovyh izvlechenij iz travy vidov cheredy metodom zhidkostnoj hromatografii [The comparative analysis of components composition of the spirit extracts from Bidens species herb by liquid chromatography method]. Vestnik farmacii. 2013;4:49–56. Russian.