WORKING OUT AND VALIDATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF LEONTODON AUTUMNALIS L.
https://doi.org/10.19163/2307-9266-2016-4-1(14)-26-35
Abstract
In the course of the study we have worked out the methodology of differential spectrophotometric determination of flavonoids in raw Leontodon autumnalis L. after the formation of a colored complex with a solution of aluminum chloride. We have established optimal conditions for complexation and extraction of flavonoids from plant raw materials under study. We have established that the most complete extraction of flavonoids is achieved with fineness of 1 mm by extraction with ethyl alcohol of 70% for 45 minutes. The maximum value of the optical density is achieved 30 minutes after addition of 2 ml of a solution of aluminum chloride 2%.
About the Authors
R. A. Bubenchikov
Kursk State Medical University, Kursk
Russian Federation
Russian Federation
Doctor of Pharmaceutical Sciences, Assistant of the Chair of Pharmacognosy and Botany at Kursk State Medical University. Area of expertise: phytochemical study of Central Black Earth Region flora and raw materials standardization
N. N. Goncharov
Kursk State Medical University, Kursk
Russian Federation
Russian Federation
postgraduate student of the Chair of Pharmacognosy and Botany at Kursk State Medical University. Area of expertise: pharmacological study of the plant from Leontodon genus
References
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Review
For citations:
Bubenchikov R.A., Goncharov N.N. WORKING OUT AND VALIDATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF LEONTODON AUTUMNALIS L. Pharmacy & Pharmacology. 2016;4(1(14)):26-35. (In Russ.) https://doi.org/10.19163/2307-9266-2016-4-1(14)-26-35
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