SURFACE-ACTIVE PROPERTIES OF THE DISTILLERS GRAINS AND THE PECTINS ISOLATED FROM THEM

Distillers grains are a huge secondary raw material resource, prospective for different fields, including pharmaceutical use, because of a rich content of biologically active compounds. The purpose of this paper was to estimate possibilities of the pharmaceutical use of distillers grains as the additive agent by means of surface-active properties of distillers grains study as well as pectins, isolated from them. We have established the surface-active properties of the liquid phase of the distillers wheat grains by means of higher pressure of air bubbles with the use of Rehbinder’s apparatus. It contained 6.67 mol/m3 pectins, and pectins isolated from a solid phase of the same distillers grains: surface activity 11.67 and 7.54, saturated surface excess amounted to 1.28×10-5 and 6.25×10-5 mol/m2, critical concentration of micelle formation amounted to 1.32 mol/m3 and 1.48 mol/m3 respectively. Surface-active characteristics of distillers grains and isolated pectins are comparable with well-known natural polysaccharides (sodium alginate, polygalacturonic acid, beet bin pectin), which are used as additive agents in technology of medicinal plants. We have determined the sizes of pectins molecules from distillers grains in the unsaturated adsorption layers, which differed from the well-known polysaccharides by lesser square (2.658 Å2), radius (0.920 Å), diameter of cross-section (1.840 Å) and the volume (2727 Å3) at the bigger mass per surface unit (1.031×10-4 кг) and a length (1026 Å). The data obtained by the sizes of pectin molecules are the favorable factor, which conduced their high biological availability. 

1. Vinarov A.Iu., Koval’skii Iu.V., Zaikina A.I. Promyshlennaia biotekhnologiia pererabotki otkhodov spirtovykh zavodov [Industrial biotechnology of alcohol plants wastes recycling]. Ekologiia okruzhaiushchei sredy stran SNG [Ecology of CIS countries environment], 2004, no. 2, pp. 84-86.

2. Izmailova V.N., Iampol’skaia G.P., Summ B.P. Poverkhnostnye iavleniia v belkovykh sistemakh [Superficial phenomena in protein systems]. Moscow, Khimiia [Chemitry], 1988, p. 184.

3. Kaishev A.Sh., Kaisheva N.Sh. Biologicheski aktivnye veshchestva otkhodov spirto-vogo proizvodstva [Biologically active substances of alcohol production wastes]. Farmatsiia i farmakologiia [Pharmacy and Pharmacology], 2014, no. 4 (5), pp. 3-22.

4. Kaishev A.Sh., Kaisheva N.Sh. Nauchnye osnovy farmatsevticheskogo ispol’zova-niia syr’evykh resursov spirtovogo proizvodstva [Scientific principles of pharmaceutical use of raw material resources of alcohol production]. Volgograd: Izdvo VolgGMU [Volgograd State Medical University publishing house], 2013, p. 156.

5. Kaishev A.Sh. Perspektivy pererabotki spirtovykh otkhodov [Prospects of alcohol wastes recycling]. Izv. vuzov. Sev.-Kav. region. Estestv. nauki [Reporter of the institutions of the North Caucasus Region. Natural sciences], 2009, no. 2 (150), pp. 76-78.

6. Kaisheva N.Sh. Issledovanie prirodnykh poliuronidov i poluchenie lekarstvennykh sredstv na ikh osnove [Study for natural polyuronides and the obtainment of the drugs on their basis]. Dis. … d-ra farmats. nauk. Piatigorsk: Piatigorskaia gosudarstvennaia farmatsevticheskaia akademiia [dissertation of the Doctor of Pharmaceutical Sciences, Pyatigorsk: Pyatigorsk State Pharmaceutical Academy], 2004, p. 369.

7. Kaisheva N.Sh. Nauchnye osnovy primeneniia poliuronidov v farmatsii [Scientific principles of the use of polyuronides in pharmacy]. Piatigorsk: PiatGFA, 2003, p. 194.

8. Pat. 2402242 Rossiiskaia Federatsiia, MPK A23L 1/30. Sposob kompleksnogo polucheniia biologicheski aktivnykh veshchestv iz spirtovykh otkhodov [Patent 2402242 Russian Federation, MPK A23L 1/30. A method of complex obtainment of biologically active substances from alcohol wastes]. N.Sh. Kaisheva, A.Sh. Kaishev (RF). No. 2008148693; applied. 09.12.08; published. 27.10.10, Biul. Izobr, no. 30, p. 9.

9. Proizvodstvenno-tekhnologicheskii reglament polucheniia spirta iz krakhmalistogo syr’ia dlia predpriiatiia OOO «Kazach’e» PTR 10-29002- 03 [Productional and technological regulations for the alcohol obtainment from starch raw material for Kazachie ltd enterprise PTR 10-29002-03]. Utv. Dep. pishch., pererab. prom-sti i detsk. pitaniia M-va sel’sk. khoz. RF 16.12.03[Approved by the Department of food and processing industry, and child nutrition, Moscow agriculture], Moscow, 2003, vol. I, p. 40.

10. Proizvodstvennyi tekhnologicheskii reglament na poluchenie spirta iz krakhmalistogo syr’ia dlia predpriiatiia OOO «Suvorovskii»[Productional technological regulations for the obtainment of alcohol from starch raw material for Suvorovski ltd enterprise]. Utv. Dep. pishch., pererab. promsti i detsk. pitaniia M-va sel’sk. khoz. RF 11.09.04 [Approved by the Department of food and processing industry, and child nutrition, Moscow agriculture], Moscow, 2004, vol. I, pp. 41.

11. Usov E.N. Kompleksnaia pererabotka poslespirtovoi bardy [Complex distillers grains processing]. Rezonans, 2006, no. 8, pp. 8–11.