DEVELOPMENT OF A SOLID DOSAGE FORM WITH ADSORPTION ACTIVITY

Enterosorbents are produced in various dosage forms – powders, tablets, pastes, etc., some of them are also manufactured in the form of capsules. A water-soluble polysaccharide complex (WSPC) manifesting a pronounced adsorption activity, which determines the prospects for the development of dosage forms of sorbents, was obtained from the cones of European Spruce (Picea abies).

The aim of the work is to develop a solid dosage form with an adsorption activity based on a water-soluble polysaccharide complex from the cones of European Spruce (Picea abies).

Materials and methods. The samples of European Spruce (Picea abies) cones were collected on the territory of Ilyinsky district of the Perm Krai and used as plant raw materials. A water-soluble polysaccharide complex was obtained from the raw materials. In order to improve the technological properties of the substance, (WSPC) granulates were obtained. The granulates were hand-made by wet granulation. The adsorption activity of the obtained granules was determined by the ability to bind methylene blue.

Results. As a result of the experiment it has been established, that the WSPC substance of European Spruce (Picea abies) cones needs to be improved in its technological properties. Granulation of the substance led to an improvement in technological properties and an increase in the adsorption activity in most of the selected compositions. It has also been shown that increased moisture content of granulate decreases its adsorption activity. A direct dependence of the adsorption activity on the concentration of the granulating liquid (with the exception of some granulates) has been revealed, but no significant effect of the size of the granulate particles on the manifestation of the adsorption effect has been reported. According to the results of the study, a dosage form “Capsules” has been proposed for the compositions that showed the best results of the adsorption activity, and their biopharmaceutical evaluation was carried out according to the disintegration test.

Conclusion. Thus, a solid dosage form with an adsorption activity has been obtained. The study shows the prospects for further research on the preparation of the drug with an adsorption activity based on the water-soluble polysaccharide complex of European Spruce (Picea abies) cones.

1. Novokshonov AA, Sokolova NV. Enterosorption method and its clinical efficacy in the complex therapy of AII in children. Questions of modern pediatrics. 2011;10(1):140- 147.

2. Hotimchenko YuS, Urmak IM, Bednyak AE, Hasina EI, Kropotov AV, Kolenchenko EA, Sergushchenko IS, Hotimchenko MYu, Kovalev VV. [Farmakologiya nekrahmal’nyh polisaharidov].Vestnik Dal’nevostochnogo otdeleniya Rossijskoj Akademii Nauk. 2005; 1:72-82. Russian

3. Kulichenko EO, Mykots LP, Tukhovskaya NA, Ligai LV, Andreeva OA, Oganesyan ET. Izuchenie adsorbcionnyh i kineticheskih harakteristik prirodnyh sorbentov po otnosheniyu k kationam svinca (II). Khimiya Rastitel’nogo syr’ya. 2019;3:335-344. https://doi.org/10.14258/jcprm.2019034595

4. Ovodov YuS. Polisaharidy cvetkovyh rastenij: struktura i fiziologicheskaya aktivnost’. Bioorganicheskaya himiya. 1998;24(7):483-501. Russian

5. Sakagami H, Kawazoe Y, Komatsu N, Simpson A, Nonoyama M, Konno K, Yoshida T, Kuroiwa Y, Tanuma S. Antitumor, antiviral and immunopotentiating activities of pine cone extracts: potential medicinal efficacy of natural and synthetic lignin-related materials (review). Anticancer Res. 1991;11(2):881-8.

6. Laksaeva, EA, Sychev IA. [Vliyanie polisaharida irgi obyknovennoj na fizicheskuyu rabotosposobnost’ zhivotnyh]. Ryazan State Medical University named after academician I.P. Pavlov. 2010;4:148-152.

7. Zlobin AA, Martinson EA, Litvinec SG, Ovechkina IA, Durnev EA, Ovodova RG. [Pektinovye polisaharidy ryabiny obyknovennoj Sorbus aucuparia L.]. Khimiya Rastitel’nogo syr’ya.2011; 1:39-44. Russian

8. Xiao H, Cai X, Fan Y, Luo A. Antioxidant Activity of Water-soluble Polysaccharides from Brasenia schreberi. Pharmacogn Mag. 2016;12(47):193-7. https://doi.org/10.4103/0973-1296.186343.

9. Halikov DH, Mirzoeva RS, Bobodzhinova GN. [O sorbcionnoj aktivnosti pektinovyh polisaharidov po otnosheniyu k ionam metallov]. Doklady Akademii nauk Respubliki Tadzhikistan. 2017;60(7-8):333-341. Russian

10. Huang B, Lu M, Wang D, Song Y, Zhou L. Versatile magnetic gel from peach gum polysaccharide for efficient adsorption of Pb2+ and Cd2+ ions and catalysis. Carbohydr Polym. 2018;181:785-792. https://doi.org/10.1016/j.carbpol.2017.11.077.

11. Tan J, Song Y, Huang X, Zhou L. Facile Functionalization of Natural Peach Gum Polysaccharide with Multiple Amine Groups for Highly Efficient Removal of Toxic Hexavalent Chromium (Cr(VI)) Ions from Water. ACS Omega. 2018;3(12):17309-17318. https://doi.org/10.1021/acsomega.8b02599.

12. Glagoleva LE, Korneeva OS, Rodinova NS, SHuvaeva GP. [Opredelenie sorbcionnyh harakteristik rastitel’nyh polisaharidnyh kompleksov v razlichnyh tekhnologicheskih sredah]. Sovremennye problemy nauki i obrazovaniya. 2012;1. Available from: http://science-education.ru/ru/article/view?id=5542. Russian

13. Sychev IA. [Mekhanizm protivovospalitel’nogo dejstviya polisaharidov donnika zheltogo]. Rossijskij mediko-biologicheskij vestnik imeni akademika I.P. Pavlova. 2008;2. [Available from]: https://cyberleninka.ru/article/n/mehanizm-protivovospalitelnogo-deystviya-polisaharidov-donnika-zheltogo. Russian

14. Wang H, Wei G, Liu F, Banerjee G, Joshi M, Bligh SW, Shi S, Lian H, Fan H, Gu X, Wang S. Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea. Int J Mol Sci. 2014;15(6):9963-78. https://doi.org/10.3390/ijms15069963.

15. Popov SV, Ovodov YuS. Polypotency of the immunomodulatory effect of pectins. Biochemistry. 2013; 78(7):1053-1067. Russian

16. Sychev IA, Podkozlin VI, Doncov VI, Kobeleva GYu, Harchenko ON. [Immunnokorregiruyushchee, antianemicheskoe i adaptogennoe dejstvie polisaharidov iz donnika lekarstvennogo]. Byulleten’ eksperimental’noj biologii i mediciny. 1996;6:661-663.

17. Bargougui K, Athmouni K, Chaieb M. Optimization, characterization and hepatoprotective effect of polysaccharides isolated from Stipa parviflora Desf. against CCl4 induced liver injury in rats using surface response methodology (RSM). Int J Biol Macromol. 2019; 132:524-533. https://doi.org/10.1016/j.ijbiomac.

18. Luo Y, Peng B, Wei W, Tian X, Wu Z. Antioxidant and Anti-Diabetic Activities of Polysaccharides from Guava Leaves. Molecules. 2019; 24(7):1343. https://doi.org/10.3390/molecules24071343.

19. Otles S, Ozgoz S. Health effects of dietary fiber. Acta Sci Pol Technol Aliment. 2014; 13(2):191-202.

20. Su H, Chen J, Miao S, Deng K, Liu J, Zeng S, Zheng B, Lu X. Lotus seed oligosaccharides at various dosages with prebiotic activity regulate gut microbiota and relieve constipation in mice. Food Chem Toxicol. 2019; 134:110838. https://doi.org/10.1016/j.fct.2019.110838.

21. Gulyaev DK, Belonogova VD, Lyalina NV. [Izuchenie sorbcionnyh svojstv polisaharidov drevesnoj zeleni i shishek eli obyknovennoj]. Sbornik nauchnyh rabot XIV Mezhdunarodnoj nauchno-prakticheskoj konferencii: «Sovremennye koncepcii nauchnyh issledovanij»]. 2015;5:64-65. Russian

22. Gulyaev DK, Lyalina NV, Rudakova IP, Belonogova VD. [Izuchenie protivovospalitel’noj aktivnosti polisaharidnyh frakcij drevesnoj zeleni i shishek eli obyknovennoj]. Sovremennye problemy nauki i obrazovaniya. - 2015;1-2. [Available fron]: http://science-education.ru/ru/article/view?id=19958. Russian

23. Gulyaev DK, Semakin DO. [Antioksidantnaya aktivnost’ polisaharidov drevesnoj zeleni i shishek eli obyknovennoj (Ricea abies (L.) H.Karst)]. Vestnik PGFA. 2017;20.161-164. Russian

24. Kochetkov NK. Himiya uglevodov. M.: Izd-vo «Himiya», 1967:672p. Russian

25. State Pharmacopoeia of the XIVed. Volume 2. Moscow: Scientific Center for the Expertise of Medicinal Products, 2018. [Available from]: http://femb.ru/femb/pharmacopea.php. Russian

26. Konorev MR. [Klinicheskaya farmakologiya enterosorbentov novogo pokoleniya]. Vestnik farmacii. 2013;4:79-85. Russian

27. Reshetnikov, VI. [Principy razrabotki lekarstvennyh form sorbentov: monografiya]. Perm’: GOU VPO PGFA Roszdrava, 2008:196 p. Russian

28. Ahnazarova SL, Kafarov VV. [Metody optimizacii eksperimenta v himicheskoj tekhnologii]. Moscow: Vyssh.shk. 1985:327 p. Russian

29. Vasilova RA, CHirkova MV, Gulyaev DK, CHugunova MP. [Issledovanie tekhnologicheskih harakteristik polisaharidnogo kompleksa shishek eli obyknovennoj]. Sbornik nauchno-prakticheskoj konf. - Vestnik PGFA, Perm’. 2018;21:168-169. Russian