INVESTIGATION OF THE ESSENTIAL OIL OF EUROPEAN SPRUCE ROOTS (LAT. PICEA ABIES H. KARST, PINACEAE FAMILY)
https://doi.org/10.19163/2307-9266-2017-5-6-520-531
Abstract
The article is devoted to the study of essential oil extracted from the roots of European Spruce (Lat. Picea abies).
The aim is to establish the component composition of the essential oil and the peculiarities of its localization in the roots of European Spruce.
Materials and methods. The objects of the study are the roots of European Spruce not longer than two centimeters in diameter, peeled and dried. The study of their anatomical signs was carried out according to the methodology of the State Pharmacopoeia of the Russian Federation (the XIII-th edition) with the”Biomed-6” microscope using the DCN 510 nozzle. The essential oil was obtained by hydrodistillation of European Spruce roots using the device of Clevenger by Method 2 of the State Pharmacopoeia of the Russian Federation (the XIII-th edition). The component composition of the essential oil was determined by an Agilent 7890A gas-liquid chromatograph with an Agilent 5975C mass-selective detector.
Results and discussion. As a result of the microscopic examination of the roots of European Spruce, it was established that the essential oil is localized mainly in resinous courses located in the wood of the root. In the central part of the root, resin moves are of a larger diameter. More than 18 components were found in the essential oil of European Spruce roots, 14 of them were identifi ed. The main component of the essential oil is sesquiterpene lactone – tanbergol.
Conclusion. The essential oil of European Spruce roots has a unique component composition that includes components not characteristic for the essential oil of spruce greenery. The difference in the component composition indicates the difference in properties and pharmacological activity. Further studies are of interest for determining the prospects of using European Spruce roots.
About the Authors
D. K. Guljaev
Perm State Pharmaceutical Academy
Russian Federation
Russian Federation
Gulyaev Dmitry Konstantinovich – PhD (Pharmacy), lecturer of the Pharmacognosy Department with a course in Botany.
2, Polevaja Str., Perm, 614990
V. D. Belonogova
Perm State Pharmaceutical Academy
Russian Federation
Russian Federation
Belonogova Valentina Dmitrievna – PhD (Pharmacy), Professor, Head of the Department of Pharmacognosy with the course of Botany.
2, Polevaja Str., Perm, 614990P. S. Mashchenko
Perm State Pharmaceutical Academy
Russian Federation
Russian Federation
Mashchenko Petr Sergeyevich – PhD (Pharmacy), docent of the Department of Toxicological Chemistry.
2, Polevaja Str., Perm, 614990
I. V. Korotkov
Perm State Pharmaceutical Academy
Russian Federation
Russian Federation
Korotkov Ilya Viktorovich – PhD (Pharmacy), docent of the Department of Pharmacognosy with a Course in Botany.
2, Polevaja Str., Perm, 614990
References
1. Lir H. Fiziologiya drevesnykh rasteniy [Physiology of woody plants]. Moscow: Mir, 1974. 422 p. Russian.
2. Mamaev SA. Formy vnutrividovoy izmenchivosti drevesnykh rasteniy [Forms of intraspecies variability of woody plants]. Moscow, 1972. 284 p. Russian.
3. Gulyaev DK, Novikova VV, Belonogova VD. Antimikrobnaya i protivogribkovaya aktivnost efi rnogo masla drevesnoy zeleni yeli obyknovennoy i yego otdel’nykh fraktsiy [Antimicrobial and antifungal activity of essential oil of woody green spruce and its individual fractions]. Medical Almanac. 2015; (4): 213–4. Russian.
4. Strukova EG, Efremov AA, Gontova AA, Sokolova LS. Vozdeystviye efi rnykh masel Sibirskogo regiona na uslovno-patogennyye mikroorganizmy [Effects of essential oils of the Siberian region on conditionally pathogenic microorganisms]. Chemistry of plant raw materials. 2009;(4):79–82. Russian.
5. Tyrkov AG, Sukhenko LT, Akmaev ER. Antimikrobnaya aktivnost efi rnykh masel, vydelennykh iz rasteniy Astrakhanskogo regiona [Antimicrobial activity of essential oils isolated from plants of the Astrakhan region]. Bulletin of Altai State Agrarian University. 2012; (2): 57–9. Russian.
6. Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem. 2005; 91(4): 621–32. DOI: 10.1016/j.foodchem.2004.06.031
7. Canillac N, Mourey А. Antimicrobial activity of the essential oil of Picea excels on Listeria, Staphylococcus aureus, and coliform bacteria. Food Microbiology. 2001: 261–8.
8. Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB. Antibacterial and antifungal effects of essential oils from coniferous trees. Biol. Pharm. Bull. 2004; 27: 863–6.
9. Sharapaeva MS, Lesovskaya MI. Vzaimosvyaz bakteritsidnykh i antioksidantnykh svoystv efi rnykh masel [Interrelation of bactericidal and antioxidant properties of essential oils]. Modern problems of science and education. [Internet]. 2011 [cited 2017 Sep 15];6. Available from: https://science-education.ru/ru/article/view?id=5067. Russian.
10. Rastitelnye resursy SSSR. Golosemennyye rasteniya, ikh khimicheskiy sostav, ispolzovaniye [Gymnosperms, their chemical composition, use]. SPb.: Nauka. 1993; (9): 465 p. Russian.
11. Stepen RA, Yesyakova OA. Vliyaniye antropogennogo zagryazneniya sredy na soderzhaniye i sostav efi rnogo masla khvoi yeli [Infl uence of anthropogenic pollution of the environment on the content and composition of essential oil of spruce needles]. Coniferous of the boreal zone. 2007; (1): 122–7. Russian.
12. Skakovsky ED, Tychinskaya LYu, Gaydukevich OA, Lamotkin SA, Vladykina DS, Rykov SV. YAMR-Analiz sostava efi rnykh masel khvoi yeli obyknovennoy v zavisimosti ot atmosfernogo zagryazneniya rayonov Minska [NMR-Analysis of the composition of essential oils of pine spruce needles, depending on the atmospheric pollution of Minsk regions]. Chemistry of plant raw materials. 2013; (3): 121–8. Russian.
13. Esyakova OA, Stepen RA. Vliyaniye zagryaznennosti vozdushnoy sredy Krasnoyarska na terpenoidnyy sostav efi rnogo masla yeli sibirskoy [Infl uence of Pollution of the Krasnoyarsk Air Environment on the Terpenoid Composition of the Siberian Spruce Essential Oil]. Chemistry of plant raw materials. 2010; (4): 139–43. Russian.
14. Vasiliev SN, Roshchin VI, Feleke C. Ekstraktivnyye veshchestva drevesnoy zeleni Picea abies (L.) Karst [Extractive substances of woody greens Picea abies (L.) Karst]. Rastitelnye resursy. 1996; (1–2): 151–76. Russian.
15. Vladykina DS, Lomatkin SA. Vozrastnaya i sezonnaya izmenchivost sostava efi rnogo masla yeli yevropeyskoy [Age and seasonal variability of essential oil composition of European spruce]. News of National Academy of Sciences of Belarus. 2014; (4): 105–8. Russian.
16. Plyashechnik MA, Aniskina AA, Loskutov SR. Sezonnoye sootnosheniye izmeneniya monoterpenov khvoi Picea obovata (Pinaceae) [Seasonal ratio of the change in monoterpenes of pine needles Picea obovata (Pinaceae)]. Rastitelnye resursy. 2011; (1): 80–6. Russian.
17. Mikhailov KL, Gushchin VA, Tarakanov AM. Organizatsiya sbora i pererabotki lesosechnykh otkhodov i drov na lesoseke [Organization of collection and processing of logging waste and fi rewood in the cutting area]. Forestry journal. 2016; (6): 98–109. Russian.
18. Chindyaev AS, Poroshilov AV. Osobennosti raspredeleniya korney Picea obovata na osushennykh nizinnykh bolotakh srednego Urala [Peculiarities of the distribution of the roots of Picea obovata on drained lowland mires of the middle Urals]. Forest bulletin. 2007;(8): 91–4. Russian.
19. Gosudarstvennaya Farmakopeya Rossijskoj Federacii XIII izdanie [State Pharmacopoeia of the Russian Federation of the XIII edition]. Vol. 2. [Internet]. Moscow, 2015. [cited 2017 Sep 10]. Available from: http://www.femb.ru/feml Russian.
20. Gulyaev DK, Anisimova AG, Belonogova VD. Anatomicheskoye i morfologicheskoye izucheniye yeli obyknovennoy shishek Piceae abietis strobili [Anatomical and morphological study of common spruce cones Piceae abietis strobili]. Medical Almanac. 2015; (1): 113–6. Russian.
21. Bercu R, Popoviciu DR. Anatomical comparative study of Larix deciduas Mill. and Picea abies (L.) Karsten (Pinaceae) leaf. Annals of R.S.C.B. 2013; (18): 172–7.
22. Gulyaev DK, Belonogova VD, Korotkov IV, Maschenko PS. Sostav efi rnogo masla drevesnoy zeleni i shishek yeli obyknovennoy, proizrastayushchey v Permskom kraye [Composition of essential oil of woody greens and cones of common spruce growing in the Perm region]. Pharmacy. 2015; (6): 14–6. Russian.
23. Gulyaev DK, Belonogova VD, Maschenko PS. Sezonnaya izmenchivost’ komponentnogo sostava efi rnogo masla drevesnoy zeleni yeli obyknovennoy Piceae abietis (Pinaceae) [Seasonal variability of the component composition of essential oil of woody greens of common Piceae abietis (Pinaceae)]. Fundamental research. 2015; (7): 14–9. Russian.
24. Gulyaev DK, Belonogova VD, Maschenko PS. Issledovaniye sezonnoy dinamiki komponentnogo sostava efi rnogo masla podrosta yeli obyknovennoy [Investigation of the seasonal dynamics of the component composition of essential oil of spruce undergrowth ordinary]. Bulletin of Perm State Pharmaceutical Academy. 2015; (15): 39–41. Russian.
25. Zule J, Tisler V, Zurej A, Niko T. Isolation and characterization of essential oils from the cones of Norway spruce (Picea abies Karst.), Europian larich (Larix decidula Mill.) and Scots pine (Pinus sylvestris). Zbornik gozdarstva in lesarstva. 2013;(71): 159–72.
26. Pharmacopoeia of the people’s republic of China (English edition 2000); (1): 7–8.
Review
For citations:
Guljaev D.K., Belonogova V.D., Mashchenko P.S., Korotkov I.V. INVESTIGATION OF THE ESSENTIAL OIL OF EUROPEAN SPRUCE ROOTS (LAT. PICEA ABIES H. KARST, PINACEAE FAMILY). Pharmacy & Pharmacology. 2017;5(6):520-531. (In Russ.) https://doi.org/10.19163/2307-9266-2017-5-6-520-531
Views:
1154
Email this article 